Chapter 1Introduction 1.1The nature of biotechnology 1.2Historical evolution of biotechnology 1.3Application of biotechnology 1.4Growth in biotechnology 1.5Strategic planning in biotechnology 1.6Summary Chapter 2Biochemistry of Growth and Metabolism 2.1Introduction 2.2Catabolism and energy 2.3Catabolic pathways 2.4Gluconeogenesis 2.5Energy metabolism in aerobic organisms 2.6Energy production in anaerobic organisms 2.7Anaerobic metabolism 2.8Biosynthesis and growth Chapter 3Applied Genetics 3.1Selection and screening 3.2Culture maintenance 3.3Mutagenesis 3.4Sexual hybridization 3.5ParasexuaL processes 3.6Recombinant DNA technology 3.7Regulation and control of recombinant DNA experimentation 3.8Summary Chapter 4Fermentation Technology 4.1The nature of fermentation 4.2Principles of microbial cultivation in aqueous systems 4.3Bioreaetor design 4.4Media design 4.5Instrumentation and process control in bioreactors 4.6Measurement techniques 4. 7Mass and energy transfer 4.8Scale-up 4.9Solid substrate fermentation 4.10Summary Chapter $Enzyme and Immobilized Cell Technology 5.1The commercial role of isolated enzymes 5.2Sources of enzymes 5.3Production of enzymes 5.4Enzyme legislation 5.5Immobilized enzymes 5.6Properties of immobilized enzymes 5.7Immobilization of cells 5.8Summary Chapter 6Downstream Processing in Biotechnology 6.1Introduction 6.2 Separation of particles 6.3Disintegration of cells 6.4Extraction methods 6.5Concentration methods 6.6Purification and resolution of mixtures 6.7Drying Chapter 7Instrumentation 7.1Introduction 7.2Terminology 7.3Process control 7.4Air flow monitoring 7.5Measurements of power input 7.6Temperature measurements 7.7Rheological measurements 7.8Foam control 7.9pH probes 7.10Redox probes 7.11Dissolved oxygen probes 7.12Enzyme probes 7.13Gas analysis 7.14Determination of cell concentration Chapter 8Processes and Products Dependent on Cultured Animal Cells 8.1Historical 8.2Types of products which can be obtained from cultured animal cells 8. 3Overview of methodology for product generation 8.4Monolayer cell growth systems 8.5Downstream processing 8.6Genetically engineered animal cells and bacteria Chapter 9Products from Plant Cells 9.1Introduction 9.2Nature of cell cultures 9.3Mass cell growth and production systems 9.4Products from cell cultures 9.5Conclusion 附录1常用前缀、后缀和构词成分 附录2常用化学、化工、生物前缀、后缀和构成成分 附录3语法、句型、词和词组的用法索引 附录4总汇词表 附录5主要参考书目
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