作者简介 赵春贞:副教授、硕士研究生导师。山乐约理学会教学与科普专业委员会副主任委员、中国药理学会教学与科普专业委员会委员、山东药理学会理事、山东毒理学会理事。山东省研究生优质课程负责人、中国医药研究生在线课程群建设项目负责人。主持、参与国家自然科学基金项目4项,发表SCI收录论文20余篇。主编、参编教材7部。授权国家发明专利3项。李成檀:副教授、硕士研究生导师,浙江省高校优秀青年教师资助计划人选。从事药理学教学16年,主讲药理学、药理学实验等课程。参编《药理学》《临床药理学》等教材、专著5部,获浙江省教学成果二等奖,主持、参与各级教学改革项目、科研项目10余项,在Ageing Res Rev、Neurobiol Aging等期刊发表论文20余篇。王雅娟:副教授、硕士研究生导师。任中国药理学会抗炎免疫药理学分会青年委员、安徽省药理学会抗炎免疫药理学专业委员会第一届委员会委员。从事教学工作近二十年,承担《药理学》临床药理学》《实验动物学》等课程的教学及相关科研工作。主持并参与了多项国家级、省级教研和科研项目,参与安徽省高等学校一流教材《护理药理学》的编写工作。
Chapter 1 General pharmacology187 Section 1?Basic knowledge of experimental animals187 Section 2?Common techniques for experimental animals192 Section 3?Ethics of experimental animals202 Section 4?Determination of plasma half life of sulfadiazine204 Section 5?Determination of LD50 of procaine210 Section 6?The influence of liver function on drug action215 Section 7?Effects of different dosage on drug action220 Section 8?Effects of different administration routes on the action of magnesium sulfate224 Section 9?Determination of IC50 of drugs in vitro228 Chapter 2 Pharmacology of efferent nervous system234 Section 1?Dose-effect relationship of acetylcholine234 Section 2?The effect of efferent nervous system drugs on isolated intestinal smooth muscle238 Section 3?The effect of efferent nervous system drugs on the pupil of rabbits243 Section 4?Inhibition of atropine on smooth muscle in mice247 Section 5?Poisoning and rescue of organophosphorus pesticides253 Chapter 3 Central nervous system pharmacology258 Section 1?Analgesia experiment of drugs258 Section 2?The effect of chlorpromazine on electric stimulation-induced agitation in mice263 Section 3?Effects of aspirin and chlorpromazine on body temperature266 Section 4?Comparison of the effects of pancuronium bromide and succinylcholine on muscle relaxation in rabbits272 Section 5?Anticonvulsant effect of phenobarbital sodium in mice276 Section 6?The effect of drugs on resisting nikethamide-induced convulsions282 Section 7?Anxiolytic effects of diazepam287 Section 8?Rescue effect of nikethamide on morphine induced respiratory depression 292 Section 9?Local and systemic effects of procaine296 Chapter 4 Cardiovascular system pharmacology301 Section 1?Effect of efferent nervous system drugs on blood pressure in rabbits301 Section 2?The effect of lidocaine on arrhythmia induced by electrical stimulation307 Section 3?The therapeutic effect of antiarrhythmic drugs on BaCl2-induced arrhythmia310 Section 4?The effect of cardiac glycosides on the relaxation and contraction of detached frog hearts 314 Section 5?Effects of propranolol on hypoxia tolerance in mice319 Chapter 5 Hormonal visceral pharmacology324 Section 1?Antagonistic effect of protamine sulfate on heparin anticoagulant activity324 Section 2?Reaction and rescue of insulin overdose328 Section 3?The antagonistic effect of aspirin on thrombosis formation332 Section 4?The effect of oxytocin on uterine smooth muscle in vitro in guinea pigs337 Section 5?The effect of diuretics on animal urine output341 Section 6?The effect of hydrocortisone on ear swelling induced by paraxylene in mice345 Section 7?Effects of magnesium sulfate and raw rhubarb on intestinal propulsion in mice350 Section 8?The effect of drugs on coagulation time in mice354 Section 9?Observation of antitussive effect of pentoverine359 Chapter 6 Pharmacology of chemotherapy drugs364 Section 1?Toxic reactions of streptomycin and its relief364 Section 2?Determination of blood concentration of cefradine367 Section 3?Hemolysis test of injection371 Section 4?Pyrogen examination of injection376 Section 5?The inhibitory effect of doxorubicin on tumor cell growth in vitro381 Section 6?The in vitro antibacterial effect of penicillin G385 Chapter 7 Experimental design392 Section 1?The objective and significance of experimental design392 Section 2?The basic elements and principles of experimental design393 Section 3?The main content and method of experimental design398 Section 4?Types of experimental design400 Section 5?The procedure and requirement of experimental design402 Chapter 8 Case analysis405 Case 1405 Case 2406 Case 3407 Case 4407 Chapter 9 Common experimental instruments409 Section 1?Information integrated signal acquisition and processing system409 Section 2?Spectrophotometer414 Section 3?Physiological and pharmacological electronic stimulators417 Appendix422 Appendix 1?Conversion of drug concentration and dosage in pharmacological experiments422 Appendix 2?Composition and preparation of common physiological solutions425 Appendix 3?Drug dosage form and prescription426 Appendix 4?Experimental report writing430
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