现代组织化学技术和方法/研究生创新教育系列教材

图书标准信息

• 作者 周劲松 编
• 出版社 西安交通大学出版社
• 出版时间 2015-04
• 版次 1
• ISBN 9787560555614
• 定价 39.80元
• 装帧 平装
• 开本 16开
• 纸张 胶版纸
• 页数 233页
• 字数 242千字
• 正文语种 简体中文，英语
• 丛书 研究生创新教育系列教材

【内容简介】

　　《现代组织化学技术和方法/研究生创新教育系列教材》的主要内容包括组织标本的前期制备技术和方法，组织化学基本概念和理论以及糖、核酸、脂和酶的染色方法，免疫组织化学基本概念和理论以及染色方法，组织化学与免疫组织化学染色技术与凝集素染色技术、PCR、原位杂交和电镜技术的结合。在《现代组织化学技术和方法/研究生创新教育系列教材》最后，着重介绍了目前比较先进的定量检测仪器的基本理论和使用方法，以及与本领域有关的最近研究进展。

【目录】

Chapter1Introduction
Section1ThecontentsandtheoriesinHistochemistry
Section2ThebasicrequirementsofHistochemistrymethods

Chapter2TheTissuePreparation
Section1Tissuecollection
Section2Fixation
Section3Tissuewashing-up，dehydrationandclearance
Section4Embedding
Section5Sectioning
Section6Adherenceandmounting
Section7Buffer

Chapter3TheCarbohydrateanditsDerivativesHistochemistry
Section1Classification
Section2Histochemistrymethods

Chapter4TheNucleicAcidHistochemistry
Section1DNAHistochemicaldemonstration
Section2ComparisondemonstrationofDNAandRNAHistochemistry

Chapter5TheLipidHistochemistry
Section1Overview
Section2Demonstrationoflipidswithphysicalmethods
Section3Demonstrationoflipidswithchemicalmethods

Chapter6EnzymeHistochemistry
Section1Enzymeanditsbasichistochemicaltheory
Section2Histochemistryforcommonenzymes

Chapter7TheBasicTheoryofImmunohistochemistry
Section1BasicImmunology
Section2Commonmarkersandtheirdetection
Section3Basicconditions

Chapter8CommonMethodsUsedinImmunohistochemistry
Section1Principles
Section2Immunofluorescencemethod
Section3Immunoenzymetechnique
Section4Avidin-biotinmethod
Section5ProteinAmethod
Section6Immunogoldmethodandimmunogold-silvermethod

Chapter9SpecificityandSensitivityofImmunohistochemistry
Section1Specificityandspecificstaining
Section2Controlexperiment
Section3Themethodstoenhanceimmunohistochemicalsensitivity

Chapter10TheDouble-StaininginImmunohistochemistry
Section1Double-stainingImmunohistochemistryonserialsection
Section2Immunofluorescencedoublelabelingtechnique
Section3Immunoenzymedoublestainingtechnique
Section4Immunoenzyme-Immunofluorescencedoublestaining
Section5Immunoenzyme-Immunogolddoublestaining

Chapter11TheLectinHistochemistry
Section1Characteristicsandapplicationoflectin
Section2ApplicationoflectinHistochemistry

Chapter12TheProgressofinSituDisplay
Section1Theenvisionmethod
Section2Thecatalyzedsignalamplificationmethod
Section3TheferricoxidealternativemethodforHRP

Chapter13InSituHybridizationImmunohistochemistry
Section1Basicprinciple
Section2Probepreparation
Section3Procedureofinsituhybridizationhistochemistry
Section4Factorsaffectinginsituhybridization
Section5Controltest

Chapter14InSituPolymeraseChainReactionTechnique
Section1Basicprinciple
Section2Basictypes
Section3Procedure
Section4ApplicationofinsituPeRtechnology

Chapter15ElectronMicroscopicCytochemicalTechnique
Section1Electronmicroscopicenzymecytochemistrytechnology
Section2ElectronmicroscopicImmunocytochemicaltechnique

Chapter16TheQuantitativeAssayofHistochemistryResults
Section1Thephotomicrography
Section2Theimageanalyzer
Section3Theflowcytometry
Section4Thelaserscanningconfocalmicroscopy
References
AppendixIPreparationofCommonlyUsedBuffer
AppendixIIHistochemistryandImmunohistochemistryExperiments
AppendixIIIFiguresandpictures