Biochemistry and Molecular Biology Laboratory Course生物化学与分子生物学实验教程（英文版）

图书标准信息

• 作者 徐跃飞、田余祥、孔英 编
• 出版社 科学出版社
• 出版时间 2014-09
• 版次 1
• ISBN 9787030418968
• 定价 48.00元
• 装帧 平装
• 开本 16开
• 纸张 胶版纸
• 页数 103页
• 字数 151千字
• 正文语种 英语
• 丛书 中国科学院教材建设专家委员会规划教材；全国高等医药院校规划教材

【内容简介】

　　《BiochemistryandMolecularBiologyLaboratoryCourse生物化学与分子生物学实验教程（英文版）》针对生物化学教学及研究中涉及的三类主要的生物分子一一蛋白质、酶和核酸，根据生物大分子的理伦性质，结合实用的电泳技术、层次技术、离心技术、分子克隆技术等，综合系统地介绍生物大分子的分离、纯化、含量测定、分析鉴定和应用，并结合教学中物质代谢、酶促反应动力学的系统分析，设计实验并融入相关实验技术和研究新进展，使学生在实验教学中掌握知识、技能的同时，体会对科研方法的选择与评价。
　　《BiochemistryandMolecularBiologyLaboratoryCourse生物化学与分子生物学实验教程（英文版）》内容新颖、科学、实用性强，可作为高等医学院校本科学生、留学生及研究生教材使用，也可供有关的教学及科研人员参考。

【目录】

Part1Basictechniquesofbiochemistryandmolecularbiology
Chapter1Basictechniquesofpreparationofbiomacromolecules
Section1Saltingout
Section2Dialysisandultrafiltration
Chapter2Spectrophotometry
Section1Basicprinciples
Section2Thebasicconstructionofspectrophotometers
Section3Somehome-madespectrophotometers
Chapter3Chromatography
Section1Adsorptionchromatography
Section2Partitionchromatography
Section3Ion-exchangechromatography
Section4Gelfiltration
Section5Affinitychromatography
Chapter4Electrophoretictechniques
Section1Basicprinciples
Section2Severaltypesofcommonlyusedelectrophoresis
Chapter5Centrifugationtechniques
Section1Basicprinciple
Section2Generaldescriptionofcentrifugation
Chapter6Geneticengineeringandanalytiealtechnologyofgeneexpression
Section1Geneticengineering
Section2Polymerasechainreaction
Section3Nucleicacidhybridization
Section4DNAsequencingtechnology

Part2Experimentsofthebiochemistryandmolecularbiology
Chapter7Analysisofpurificationandphysicochemicalpropertiesofproteins
Experiment1Colorimetricmethodsforproteindetermination
Experiment2Isolationandpurificationofserumy-globulin
Experiment3Celluloseacetatemembraneelectrophoresisofserumproteins
Experiment4DeterminationofrelativemolecularmassofproteininSDS-PAGE
Chapter8Kineticsofenzyme-catalysedreactions
Experiment1Thespecificityofenzymeandeffectoftemperature，pHontheenzymeActivity
Experiment2Isolationofalkalinephosphataseanddeterminationofitsspecificactivity
Experiment3Effectsofsubstratesconcentrationofenzymaticactivity：determinationofKmofalkalinephosphatase
Experiment4Inhibitioneffectofphosphateonalkalinephosphatase
Experiment5Analysisoflacticdehydrogenaseisoenzyme
Chapter9Substancemetabolism
Experiment1Extractionandidentificationofliverglycogen
Experiment2Determinationofalaninetransaminaseactivity（Reitman-Frankels'method）
Experiment3Enzymecatalyzedtransaminationofaminoacidsdetectedbypaperchromatography
Chapter10Basicexperimentofmolecularbiology
Experiment1PreparationofthegenomicDNAintheeukaryoticcells
ExPeriment2DeterminationoftheconcentrationandpurityofDNAbyspectroscopy
Experiment3Agarosegelelectrophoresisofnucleicacids
Experiment4Polymerasechainreaction（PCR）
Experiment5IsolationandpurificationoftheDNAfragments
Experiment6DNAligation——constructingrecombinantplasmids
Experiment7PreparationofE.colicompetentcells
Experiment8TransformationofCa2+competentceils
Experiment9ThescreeningofrecombinantDNA
Experiment10MinipreparationofplasmidDNA
Experiment11DigestionofplasmidDNAwithrestrictionendonucleases
Experiment12Southernblot
Experiment13Expressionofexogenousgeneinprokaryoticcells
References

Appendix
AppendixIThecalculationtableofammoniumsulfatesaturation
AppendixIICommonbuffersolutionpreparation
AppendixIIITherelativemolecularmassandpIofprotein
AppendixIVCommondataofchromatography
AppendixVTheconversionbetweencentrifugationspeedofcentrifugationforce