Experimental Manual in Medical Biochemistry

图书标准信息

• 作者 YU、Huang、Chief Editors 著
• 出版社 武汉大学出版社
• 出版时间 2008-09
• 版次 1
• ISBN 9787307065345
• 定价 22.00元
• 装帧 平装
• 开本 16开
• 纸张 胶版纸
• 页数 194页
• 字数 283千字

【内容简介】

《EXPERIMENTALMANUALINMEDICALBIOCHEMISTRY》isprimarilyintendedforundergraduatemedicalstudents,andhopefullyitwillbeausefulreferencebookforgraduatestudentsandteachingstaff.Theobjectivesforthecourseareforstudentstolearnmodernbiochemicallaboratorymethodologiesandtechniques,developtheabilitytoperformexperimentssuccessfullyandindependently,properlyinterpretthedataandwritescientificlaboratoryreports.Thefirsteditionofthismanualpresentsshortenedversionsofthebasicbiochemistrymethodsdesignedforuseatthelabbenchinabiochemistryandmolecularbiologylaboratory.Studentswholearnbiochemistryasapartofmedicalscienceshouldbeabletounderstandtheconceptsandmethodsofnotonlytheconventionalclinicallaboratorytestsbutalsotherecentlydevelopedtests.Forthispurpose,wehavemadethismanualwithincorporationofsomeclinicallaboratorytestkits.Alloftheexperimentshavebeenperformedmanytimesinourownlaboratory.Wehopethattheywillprovideareliabletrainingofthemostimportantandcommonlyusedtechniquesofcontemporarybiochemistry.
WeexpressourappreciationtoourcolleaguesintheDepartmentofBiochemistryandMolecularBiology,WuhanUniversitySchoolofMedicine,whohavecontributedideas,techniquesandexperiencestothemanual.Wearereallygratefultoalltheexperts,especiallythosefromotheruniversities,whomadebigcontributiontothecompilingofvariouschapters.

【目录】

ChapterⅠSpectrophotometry
1.1BasicConceptsandPrincipleofSpectrophOtometry
1.2MeasurementofLightAbsorption
1.3AplicationsofSpectrophotometry
EXP.1QuantitativeAnalysisofProtein
EXP.2DeterminationofBloodGlucose
ChapterⅡElectrophoresis
2.1BasicPrinciplesofElectrophoresis
2.2ImpactFactorsofElectrophoresis
2.3DetectionofComponentsafterElectrophoreticSeparation
2.4SpecialElectrophoreticTechniquesandApplications
EXP.3SeparationofSerumProteinsbyCelluloseAcetateMembraneElectrophoresis
EXP.4SeparationofSerumLipoproteinsbyAgaroseGelElectrophoresis
EXP.5ProteinsSeparationbySDS—PolyacrylamideGelElectrophoresis
ChapterⅢChromatography
3.1FundamentalPrinciplesofChromatography
3.2CommonlyUsedChromatographiCTechniques
EXP.6DetectingTransaminationofAminoAcidsbyPaperChromatography
EXP.7SeparationofMixedAminoAcidsbyCationExchangeChromatography
EXP.8SeparationofHemoglobinandDinitrophenyl(DNP)-glutamatebyGelFiltrationChromatography
ChapterⅣEnzymeAnalysis
4.1TheActivityofEnzyme
4.2Enzymekinetics(Factorsaffectingreactionvelocity)
4.3Enzvmarieanalysis
EXP.9AssaytheActivityofAlanineAminotransferase(ALT)inSerum(Mohun’SMethod)
EXP.10LactateDehydrogenase(LDH)Analysis
EXP.11EffectofSubstrateConcentrationonEnzymeActivity.DeterminingKmValueforAlkalinePhosphatase
EXP.12Coupledenzymaticreactionassay(EnzymaticEndpointMethod)-DeterminationofTotalCholesterol，Triglyceridesand
GlucoseinSerum
ChapterⅤIsolationandPurificationofProtein
5.1MethodsandBasicPrinciplesofProteinPurification
5.2ProcedureofProteinPreparation
5.3Crystallization，Concentration，DryingandStorageofProtein
5.4IdentificationandAnalysisofProtein
EXP.13IsolationandIdentificationofSerumIgG
EXP.14Expression.PurificationandIdentificationofGlutathioneS.TransferaseFusionProtein
ChapterⅥIsolation.PurificationandIdentificationofNucleicAcids
6.1IsolationandPurificationofNucleicAcids
6.2IdentificationandAnalysisofNucleicAcids
EXP.15IsolationofEukaryoticGenomicDNAbyProteinaseK.PhenolorNaIMethod
EXP.16SDS.AlkalineLysisofPlasmidDNA
EXP.17IsolationofTotalRNAbyTRIzolReagent
……
ChapterⅦGeneticEngineering
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