目录 前言 第l部分 生物化学实验基本原理与方法 Part l Basic Principles and Methodologies of Biochemical Experiments I生物大分子的分离与纯化的一般步骤与方法 I General Procedures and Methods for Isolation and Purification of Biomacromolecules 11分光光度法 II Spectrophotometry III层析 III Chromatography IV电泳 ⅣElectr叩hOresis V离心 V Centrifugation V1分子生物学技术基础――聚合酶链式反应(PcR) VI Brief Introduction to Basic Technique of Molecular BiologyPolymerase Chain Reaction(PCR、 第2部分 基础性实验 Part 2 Basic Experiments 实验1蛋白质和氨基酸的呈色反应 Experiment l Color Reactions of Proteins and Amino Acids 实验2蛋白质的沉淀与变性 Experiment 2 Precipitation and Denaturation ofProteins 实验3微量凯氏定氮法测蛋白质总氮含量 Experiment 3 Quantification of Crude Proteins―Kieldahl’S Nitrogen Determination 实验4双缩脲法测定蛋白质含量 Experiment 4 Quantification ofProteins―Biulet Method 实验5 Folin一酚试剂法测定蛋白质含量 Experiment 5 Quantification ofProteins―――Folin―Lowry Method 实验6紫外吸收法测定蛋白质含量 Experiment 6 Quantification ofProteins by Ultraviolet Absorption 实验7染料结合法测定蛋白质含量 Experiment 7 Quantification ofProteins by Dye―binding Method 实验8几种重要氨基酸(赖氨酸、色氨酸和脯氨酸)的含量测定 Experiment 8 Determination of the Contents of Some Important Amino Acids (LysTrp and Pro) 实验9血清蛋白的醋酸纤维薄膜电泳 Experiment 9 Cellulose Acetate Membrane Electrophoresis of Serum Proteins 实验10酪蛋白的制备 Experiment 10 Preparation ofCasein 实验11 SDS聚丙烯酰胺凝胶电泳(PAGE)~IJ定蛋白质分子质量 Experiment 11 Molecular Weight Determination ofProteins by SDSPAGE 实验12聚丙烯酰胺凝胶等电聚焦测定蛋白质等电点 Experiment 12 pI Determination of Proteins by Polyacrylamide Gel Isoelectrofocusing 实验13酶的特性 Experiment 13 Properties ofEnzymes 实验14多酚氧化酶米氏常数(‰)的测定 Experiment 14 Determination of Michaelis Constant(Kin)of Polyphenol Oxidase 实验1 5核酸酶的活力测定 Experiment 15 Activity Assay ofNucleases 实验16谷丙转氨酶活力测定 Experiment 16 Determination ofActivity of Glutamate―Pyruvate Transaminase 实验l 7植物抗氧化酶类(超氧物歧化酶、过氧化物酶和过氧化氢酶)活 性测定 Experiment 17 Activity Assay of Common Antioxidant Enzymes(SOD,POD and CAT)In Plant 实验1 8乳酸脱氢酶(LDH)活性测定 Experiment 18 Activity Assay ofLactate Dehydrogenase(LDH) 实验19维生素C的定量测定――2,6二氯酚靛酚法 Experiment 19 Quantification ofVitamin C by 2,6Dichlorophenolindo Phenol Method 实验20花菜中核酸的分离和鉴定 Experiment 20 Extraction and Identification ofNucleic Acids From Cauliflower 实验21酵母核糖核酸的分离及组分鉴定 Experiment 21 Extraction ofYeast RNA and Identification Of Its Components 实验22定磷法测定核酸含量 Experiment 22 Quantification of Nucleic Acids by Method of Phosphate Determination 实验23定糖法测定RNA含量 Experiment 23 Quantification of RNA by Ribose Determination Method 实验24二苯胺法测定DNA含量 Experiment 24 Quantification of DNA by Diphenylamine Method 实验25紫外吸收法测定核酸含量 Experiment 25 Quantification ofNucleic Acids by Ultraviolet Absorption Method 实验26醋酸纤维薄膜电泳分离核糖核苷酸 Experiment 26 Separation of Ribonucleotides by Cellulose Acetate Membrane Electrophoresis 实验27 还原糖和总糖的含量测定_DNs法 Experiment 27 Determination of Reducing Sugar and Total Sugar by DNS Method 实验28血糖的定量测定――葡萄糖氧化酶氨基安替比林(COD―PAP) Experiment 28 Quantification of Blood Glucose by Glucose Oxidase4 Aminoantipyrine(GOD―PAP) 实验29粗脂肪的提取和测定 Experiment 29 Extraction and Quantitative Determination ofCrude Fat 实验30 油脂的化学性质 Experiment 30 Chemical Properties ofOil or Fat 第3部分 综合性实验 Part 3 Comprehensive Experiments 实验3l蛋白质的酸水解及其氨基酸的纸层析鉴定 Experiment 3 1 Acid Hydrolysis of Proteins and Isolation and Identification ofAmino Acids by Paper Chromatography 实验32离子交换层析分离混合氨基酸 Experiment 32 Separating Mixed Amino Acids by Ion―exchange Chromatography 实验33血清球蛋白的盐析分离及分子筛凝胶层析法脱盐 Experiment 33 Saltingout Separation of Serum Globulins and Desalting by Molecular Sieving Gel Chromatography 实验34糖的测定和鉴定――蒽酮试剂和纸层析法 Experiment 34 Quantification and Identification of Saccharides by Anthrone Colorimetry and Paper Chromatography 实验35聚丙烯酰胺凝胶电泳分离乳酸脱氢酶同工酶 Experiment 35 Separating LDH Isoenzymes by PAGE 实验36叶绿体的制备及相关分析 Experiment 36 Preparation and Anabrses ofChloroplasts 实验37植物分子生物学系列实验 Experiment 37 Series Experiments ofPlant Molecular Biology 参考文献 附录 附录1 基础生物化学实验室常用仪器简介 附录2 常用缓冲溶液的配制
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