基因克隆和DNA分析
￥ 27.91 5.9折 ￥ 47 九五品
仅1件
作者[英]T.A.Brown
出版社高等教育出版社
ISBN9787040489132
出版时间2018-01
版次1
装帧平装
开本16开
纸张胶版纸
页数353页
定价47元
上书时间2024-06-01
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基本信息
书名:基因克隆和DNA分析
定价：47.00元
作者:[英]T.A.Brown
出版社：高等教育出版社
出版日期：2018-01-01
ISBN：9787040489132
字数：
页码：353
版次：
装帧：平装
开本：16开
商品重量：
编辑推荐
内容提要
《基因克隆和DNA分析（第7版 ）》的英文原版是一本在国外很有影响，且使用广泛的基因克隆和DNA分析的入门教材。全书深入浅出地阐述了分子生物学基本技术如基因克隆、基因表达、PCR、基因组学等的原理，同时又生动地介绍了基因克隆和DNA分析在基础研究、医学、农学、法医学等领域中的实际应用。 《基因克隆和DNA分析（第7版 ）》适合作为高等院校生物学、农林、医药类专业的教学参考书，也可供生命科学相关科研人员和中学生物教师参考。
目录
作者介绍
序言
Preface to the Seventh EditionAbout the Companion WebsitePart 1 The Basic Principles of Gene Cloning and DNA Analysis1 Why Gene Cloning and DNA Analysis are Important1．1 The early development of genetics1．2 The advent of gene cloning and the polymerase chain reaction1．3 What is gene cloning?1．4 What is PCR?1．5 Why gene cloning and PCR are so important1．5．1 Obtaining a pure sample of a gene by cloning1．5．2 PCR can also be used to purify a gene1．6 How to find your way through this bookFurther reading 122 Vectors for Gene Cloning： Plasmids and Bacteriophages2．1 Plasmids2．1．1 Size and copy number2．1．2 Conjugation and compatibility2．1．3 Plasmid classification2．1．4 Plasmids in organisms other than bacteria2．2 Bacteriophages2．2．1 The phage infection cycle2．2．2 Lysogenic phagesGene organization in the □ DNA moleculeThe linear and circular forms of □ DNAM13-a filamentous phage2．2．3 Viruses as cloning vectors for other organismsFurther reading3 Purification of DNA from Living Cells3．1 Preparation of total cell DNA3．1．1 Growing and harvesting a bacterial culture3．1．2 Preparation of a cell extract3．1．3 Purification of DNA from a cell extractRemoving contaminants by organic extraction and enzyme digestionUsing ion-exchange chromatography to purify DNA from a cell extractUsing silica to purify DNA from a cell extract3．1．4 Concentration of DNA samples3．1．5 Measurement of DNA concentration3．1．6 Other methods for the preparation of total cell DNA3．2 Preparation of plasmid DNA3．2．1 Separation on the basis of size3．2．2 Separation on the basis of conformationAlkaline denaturationEthidium bromide-caesium chloride density gradient centrifugation3．2．3 Plasmid amplification3．3 Preparation of bacteriophage DNA3．3．1 Growth of cultures to obtain a high □ titre3．3．2 Preparation of non-lysogenic □ phages3．3．3 Collection of phages from an infected culture3．3．4 Purification of DNA from □ phage particles3．3．5 Purification of M13 DNA causes few problemsFurther reading4 Manipulation of Purified DNA4．1 The range of DNA manipulative enzymes4．1．1 Nucleases4．1．2 Ligases4．1．3 Polymerases4．1．4 DNA-modifying enzymes4．2 Enzymes for cutting DNA： Restriction endonucleases4．2．1 The discovery and function of restriction endonucleases4．2．2 Type II restriction endonucleases cut DNA at specific nucleotide sequences4．2．3 Blunt ends and sticky ends4．2．4 The frequency of recognition sequences in a DNA molecule4．2．5 Performing a restriction digest in the laboratory4．2．6 Analysing the result of restriction endonuclease cleavageSeparation of molecules by gel electrophoresisVisualizing DNA molecules in an agarose gel4．2．7 Estimation of the sizes of DNA molecules4．2．8 Mapping the positions of different restriction sites in a DNA moleculePart Ⅱ The Applications of Gene Cloning and DNA Analysis in ResearchPart Ⅲ The Applications of Gene Cloning and DNA Analysis in BiotechnologyGlossaryIndex
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