Des Nicholl presents here a new, fully revised, and expandededition of his popular undergraduate-level textbook. Many of thefeatures of the original edition have been retained; the book stilloffers a concise technical introduction to the subject of geneticengineering. However, the book is now divided into three mainsections: the first introduces students to basic molecular biology,the second section explains the methods used to manipulate genes,and the third deals with modern applications of geneticengineering. A whole chapter is now devoted to the polymerase chainreaction. Applications covered in the book include genomics,protein engineering, gene therapy, cloning, and transgenic animalsand plants. A final chapter discusses the ethical questionssurrounding genetic engineering in general. An Introduction toGenetic Engineering is essential reading for undergraduate studentsof biotechnology, genetics, molecular biology and biochemistry.
【目录】
Preface to the second edition
1. Introduction
Part I. The Basis of Genetic Engineering:
2. Introducing molecular biology
3. Working with nucleic acids
4. The tools of the trade
Part II. The Methodology of Gene Manipulation:
5. Host cells and vectors
6. Cloning strategies
7. The polymerase chain reaction
8. Selection, screening, and analysis of recombinants
Part III. Genetic Engineering in Action:
9. Understanding genes and genomes
10. Genetic engineering and biotechnology
11. Medical and forensic applications of gene manipulation
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