国外化学名著系列8（影印版）：荧光分析法原理

图书标准信息

• 作者 [美]拉科维兹 著
• 出版社 科学出版社
• 出版时间 2008-03
• 版次 1
• ISBN 9787030211880
• 定价 138.00元
• 装帧 精装
• 开本 16开
• 纸张 胶版纸
• 页数 954页
• 字数 1200千字
• 正文语种 英语
• 丛书 国外化学名著系列8

【内容简介】

　　本书在广受欢迎的前几版基础上修订而成。第三版保持了其侧重于基本概念的优点，更新了最新文献成果，增加了新的章节。主要内容包括：荧光仪器，荧光闭，荧光寿命，溶剂和环境效应，猝灭及其机理与动力学，各向异性，能量传输，蛋白质荧光，多光子激发与显微镜，传感器，DNA技术，单分子检测，荧光相关光谱，新型探针和辐射衰变工程等。
　　本书可供分析化学、生物物理、生物化学、生物工程、生物学和医学等专业高年级本科生、研究生、科研人员参考。

【目录】

1IntroductiontoFluorescence
1.1PhenomenaofFluorescence
1.2JablonskiDiagram
1.3CharacteristicsofFluorescenceEmission
1.3.1TheStokesShift
1.3.2EmissionSpectraAreTypicallyIndependentoftheExcitationWavelength
1.3.3ExceptionstotheMirror-ImageRule
1.4FluorescenceLifetimesandQuantumYields
1.4.1FluorescenceQuenching
1.4.2TimescaleofMolecularProcessesinSolution
1.5FluorescenceAnisotropy
1.6ResonanceEnergyTransfer
1.7Steady-StateandTime-ResolvedFluorescence
1.7.1WhyTime-ResolvedMeasurements?
1.8BiochemicalFluorophores
1.8.1FluorescentIndicators
1.9MolecularInformationfromFluorescence
1.9.1EmissionSpectraandtheStokesShift
1.9.2QuenchingofFluorescence
1.9.3FluorescencePolarizationorAnisotropy
1.9.4ResonanceEnergyTransfer
1.10BiochemicalExamplesofBasicPhenomena
1.11NewFluorescenceTechnologies
1.11.1MultiphotonExcitation
1.11.2FluorescenceCorrelationSpectroscopy
1.11.3Single-MoleculeDetection
1.12OverviewofFluorescenceSpectroscopy
References
Problems
2InstrumentationforFluorescenceSpectroscopy
2.1Spectrofluorometers
2.1.1SpectrofluorometersforSpectroscopyResearch
2.1.2SpectrofluorometersforHighThroughput
2.1.3AnIdealSpectrofluorometer
2.1.4DistortionsinExcitationandEmissionSpectra
2.2LightSources
2.2.1ArcLampsandIncandescentXenonLamps
2.2.2PulsedXenonLamps
2.2.3High-PressureMercury(Hg)Lamps
2.2.4Xe-HgArcLamps
2.2.5Quartz-TungstenHalogen(QTH)Lamps
2.2.6Low-PressureHgandHg-ArLamps
2.2.7LEDLightSources
2.2.8LaserDiodes
2.3Monochromators
2.3.1WavelengthResolutionandEmissionSpectra
2.3.2PolarizationCharacteristicsofMonochromators
2.3.3StrayLightinMonochromators
2.3.4Second-OrderTransmissioninMonochromators
2.3.5CalibrationofMonochromators
2.4OpticalFilters
2.4.1ColoredFilters
2.4.2Thin-FilmFilters
2.4.3FilterCombinations
2.4.4Neutral-DensityFilters
2.4.5FiltersforFluorescenceMicroscopy
2.5OpticalFiltersandSignalPurity
2.5.1EmissionSpectraTakenthroughFilters
2.6PhotomultiplierTubes
2.6.1SpectralResponseofPMTs
2.6.2PMTDesignsandDynodeChains
2.6.3TimeResponseofPhotomultiplierTubes
2.6.4PhotonCountingversusAnalogDetectionofHuorescence
2.6.5SymptomsofPMTFailure
2.6.6CCDDetectors
2.7Polarizers
2.8CorrectedExcitationSpectra
2.8.1CorrectedExcitationSpectraUsingaQuantumCounter
2.9CorrectedEmissionSpectra
2.9.1ComparisonwithKnownEmissionSpectra
2.9.2CorrectionsUsingaStandardLamp
2.9.3CorrectionFactorsUsingaQuantumCounterandScatterer
2.9.4ConversionbetweenWavelengthandWavenumber
2.10QuantumYieldStandards
2.11EffectsofSampleGeometry
2.12CommonErrorsinSamplePreparation
2.13AbsorptionofLightandDeviationfromtheBeer-LambertLaw
2.13.1DeviationsfromBeersLaw
2.14Conclusions
References
Problems
3Fluorophores
3.1IntrinsicorNaturalFhiorophores
3.1.1FluorescenceEnzymeCofactors
3.1.2BindingofNADHtoaProtein
3.2ExlrinsieFluorophores
3.2.1Protein-LabelingReagents
3.2.2RoleoftheStokesShiftinProteinLabeling
3.2.3PhotostabilityofFluorophores
3.2.4Non-Covalentprotein-LabelingProbes
3.2.5Membraneprobes
3.2.6MembranePotentialProbes
3.3RedandNear-Infrared(NIR)Dyes
3.4DNAProbes
3.4.1DNABaseAnalogues
3.5ChemicalSensingProbes
3.6SpecialProbes
3.6.1FluorogenieProbes
3.6.2StructuralAnaloguesofBiomolecules
3.6.3ViscosityProbes
3.7GreenFluorescentProteins
3.8OtherFluorescentProteins
3.8.1Phytofluors:ANewClassofFluorescentProbes
3.8.2Phycobiliproteins
3.8.3SpecificLabelingofIntraeelhilarProteins
3.9Long-Lifetimeprobes
3.9.1Lanthanides
3.9.2TransitionMetal-LigandComplexes
3.10ProteinsasSensors
3.11Conclusion
References
problems
4Time-DomainLifetimeMeasurements
4.1OverviewofTime-DomainandFrequency-
DomainMeasurements
4.1.1MeaningoftheLifetimeorDecayTime
4.1.2PhaseandModulationLifetimes
4.1.3ExamplesofTime-DomainandFrequency-DomainLifetimes
4.2BiopolymersDisplayMulti-ExponentialorHeterogeneousDecays
4.2.1ResolutionofMulti-ExponentialDecaysIsDifficult
4.3Time-CorrelatedSingle-PhotonCounting
4.3.1PrinciplesofTCSPC
4.3.2ExampleofTCSPCData
4.3.3ConvolutionIntegral
4.4LightSourcesforTCSPC
4.4.lLaserDiodesandLight-EmittingDiodes
4.4.2FemtosecondTitaniumSapphireLasers
4.4.3PicosecondDyeLasers
4.4.4Flashlamps
4.4.5SynchrotronRadiation
4.5ElectronicsforTCSPC
4.5.1ConstantFractionDiscriminators
4.5.2Amplifiers
4.5.3Time-to-AmplitudeConverter(TAC)andAnalyte-to-DigitalConverter(ADC)
4.5.4MultichannelAnalyzer
4.5.5DelayLines
4.5.6PulsePile-Up
4.6DetectorsforTCSPC
4.6.1MicrochannelPlatePMTs
4.6.2DynodeChainPMTs
4.6.3CompactPMTs
4.6.4PhotodiodesasDetectors
4.6.5ColorEffectsinDetectors
4.6.6TimingEffectsofMonochromators
4.7Multi-DetectorandMultidimensionalTCSPC
4.7.1MultidimensionalTCSPCandDNASequencing
4.7.2DeadTimesRepetitionRatesandPhotonCountingRates
4.8AlternativeMethodsforTime-Resolved
Measurements
4.8.1TransientRecording
4.8.2StreakCameras
4.8.3UpconversionMethods
4.8.4MicrosecondLuminescenceDecays
4.9DataAnalysis:NonlinearLeastSquares
4.9.1AssumptionsofNonlinearLeastSquares
4.9.2OverviewofLeast-SquaresAnalysis
4.9.3MeaningoftheGoodness-of-Fit
4.9.4AutocorrelationFunction
4.10AnalysisofMulti-ExponentialDecays
4.10.1p-TerphenylandIndole:TwoWidely
SpacedLifetimes
4.10.2ComparisonofXR2Values:FStatistic
4.10.3ParameterUncertainty:Confidence
Intervals
4.10.4EffectoftheNumberofPhotonCounts
4.10.5AnthranilicAcidand2-Aminopurine:
TwoCloselySpacedLifetimes
……
5Frequency-DomainLifetimeMeasurements
6SolventandEnvironmentalEffects
7DynamicsofSolventandSpectralRelaxation
8QuenchingofFluourescence
9MechanismsandDynamicsofFluorescenceQuenching
10FluorescenceAnisotropy
11Time-DependentAnisotropyDecays
12AdvancedAnisotropyConcepts
13EnergyTransfer
14Time-ResolvedEnergyTransferandConformationalDistributionsofBiopolymers
15EnergyTransfertoMultipleAcceptorsinOne,Two,orThreeDimensions
16ProteinFluorescence
17Time-ResolvedProteinFluorescence
18MultiphotonExcitationandmicrosocopy
19FluorescenceSensing
20NovelFluorophores
21DNATechnology
22Fluorescence-LifetimeImagingMicroscopy
23Single-MoleculeDetection
24FluorescenceCorrelationSpectroscopy
25RadiativeDecayEngineering:Metal-EnhancedFluorescence
26RadiativeDecayEngineering:SurfacePlasmon-CoupledEmission
AppendixI.CorrectedEmissionSpectra
AppendixII.FluorescentLifetimeStandards
AppendixIII.AdditionalReading
AnswerstoProblems
Index