新型纳米金蛋白质定位微试管芯片和计算与信号转导

图书标准信息

• 作者 王琳 著
• 出版社 清华大学出版社
• 出版时间 2008-09
• 版次 1
• ISBN 9787302178309
• 定价 80.00元
• 装帧 精装
• 开本 16开
• 纸张 胶版纸
• 页数 135页
• 字数 201千字
• 正文语种 英语

【内容简介】

《新型纳米金蛋白质定位微试管芯片和计算与信号转导》作者原创一种新型纳米金微芯片，并整合细胞核和细胞质蛋白的纯化结果和生物计算技术（如DB索引算法，由距离矩阵、色彩相似性等决定聚类边界），通过分子标注和击打次数，建立细胞信号转导模型。该系统具有高特异性、高敏感性、高重复性、高稳定性、高信号性、样本检测量大、简便性、安全性、多参数实时定量的特点，通过计算后可建立分子机制网络。

【目录】

Chapter1Introduction
1.1AntibodyArrayTechnologiesandApplications
1.2ProteinPhosphorylation,LocalizationandFunction
1.3InteractionProteomicsandPathwayBuilding
1.3.1BMP2Signaling
1.3.2TheEffectofSTI571onCellSignaling

Chapter2Technologies
2.1Materials
2.1.1CellLines
2.1.2Chemicals
2.1.3Antibodies
2.1.4Protein-MolecularWeightMarker
2.1.5Apparatus
2.2Methods
2.2.1CellCulture
2.2.2CellNumbersandExperimentalFormat
2.2.3CytoplasmicandNuclearProteinExtracts
2.2.4DeterminationofProteinConcentration
2.2.5PonceauSStaining
2.2.6WesternAnalysis
2.2.7Immunoprecipitation
2.2.8CellCycleAnalysis
2.3SilverStaininginArrayTube
2.3.1BiotinylationofCellularProteins
2.3.2IncubationofProteinExtracts
2.3.3SilverStaining
2.3.4StatisticalAnalysis

Chapter3NewCytoplasmicandNuclearBMP2-SignalingPatterninU937Cells
3.1ModulationofPhosphorylationandLocalizationinBMP2-TreatedU937Cells
3.1.1Totalp-Tyrosineandp-p38,p38Increased:IncreasingCytoplasmandNucleus
3.1.2Totalp-ERKandp-JNKIncreased:IncreasingCytoplasmandUnchangedNucleus
3.1.3Totalp-AktIncreased:IncreasingCytoplasmandDecreasingNucleus
3,1.4Totalp-Smadl,p-Smad2/3,c-Mycandp-STAT3Unchanged:DecreasingCytoplasmandIncreasingNucleus
3.1.5Totalp-p70S6Unchanged:IncreasingCytoplasmandDecreasingNucleus
3.2BMP2SignalinginU937Cells
3.2.1BMP2ActivatesAkt,ERKandINKPathwayinU937Cells
3.2.2BMP2ActivatesSmadlandSmad2/3Networks,p38Networkandc-Myc,TyrosineNetworkinU937Cells
3.2.3BMP2Inhibitsp70S6SignalinginU937cells
3.3BMP2SignalingModelinU937Cells

Chapter4NewCytoplasmicandNuclearBMP2-SignalinginMCF7Cells
4.1ModulationofPhosphorylationandLocalizationinBMP2-TreatedMCF7Cells
4.1.1Totalp-JNK,p-Smadl,p-Smad2/3,p-p38andp38Unchanged:IncreasingCytoplasmandDecreasingNucleus
4.1.2p-Tyrosinc,p-STAT3,p-ERKandp-p70S6Increased:IncreasingCytoplasmandUnchangedNucleus
4.1.3Totalc-MycUnchanged:SlightlyIncreasingNucleusandSlightlyDecreasingCytoplasm
4.1.4Totalp-AktUnchanged:UnchangedNucleusandCytoplasm
4.2BMP2SignalinginMCF7Cells
4.2.1BMP2Activatesp70S6andERKSignaling,TyrosineandSTAT3NetworkinMCF7Cells..
4.2.2BMP2Inhibitsp38,JNKandSmadSignalinginMCF7Cells
4.2.3BMP2Activatesc-MycSignalinginMCF7Cells
4.3BMP2SignalingModelinMCF7Cells

Chapter5NewCytoplasmicandNuclearST1571SignalinginK562Cells
5.1ModulationofPhosphorylationandLocalizationinSTI571-TreatedK562Cells
5.1.1Totalp-Aktandp-p70S6Decreased:DecreasingCytoplasmandNucleus
5.1.2Totalp-p38,p38,c-Myc,p-Tyrosine,p-Smadlandp-Smad2/3Increased:IncreasingCytoplasmandNucleus
5.1.3Totalp-STAT3andp-JNKIncreased:IncreasingCytoplasmandUnchangedNucleus
5.1.4Totalp-ERKIncreased:DecreasingCytoplasmandIncreasingNucleus
5.2STI571SignalinginK562Cells
5.2.1STI571InhibitsAkt/p70S6PI3KSignalinginK562Cells
5.2.2STI571Activatesp38MAPKPathway,c-Myc,TyrosineandSmadI/Smad2/Smad3NetworksinK562Cells
5.2.3ST[571ActivatesERKPathwayinK562Cells
5.2.4ST1571ActivatesSTAT3NetworkandJNK/MAPKPathwayinK562Cells
5.3STI571SignalingModelinK562Cells

Chapter6PositiveandNegativeCo-localizedRelationshipsinThreeTreatedCellLines
6.1PositiveandNegativeCo-localizedDistributions
6.1.1p-p38andp38
6.1.2SmadlandSmad2/3
6.1.3Akt,ERKandp70S6
6.1.4p70S6withp38andSmad1,2,3
6.1.5ERKorp38withSmadl
6.1.6ERKorp38withc-Myc
6.2PossibleRelationships

Chapter7CytoplasmicSignalinginThreeTreatedCellLines
7.1CytoplasmicDistribution
7.1.1p-ERK,p-JNKandp-AktinBMP2-InducedApoptosisinU937Cells
7.1.2p-STAT3andp-JNKinSTI571-TreatedK562Cells
7.1.3p-Tyrosine,p-STAT3,p-ERKandp-p70S6inBMP2-TreatedMCF7Cells
7.2Co-cytoplasmicSignaling

Chapter8ProteomicSilencinginTreatedHumanCancerCellLines
8.1ProteomicSilenceDistributions
8.1.1p-p70S6NuclearDecreaseinBMP2-InducedApoptosisinU937Cells
8.1.2p-ERK/p-JNK/p-p38,p-Smadlandp-Smad2/3Co-nuclearDecreaseinBMP2-TreatedMCF7Cells
8.1.3p-Aktandp-p70S6Co-cytoplasmicandCo-nuclearDecreaseinSTI571-TreatedK562Cells
8.2PossibleRelationships

Chapter9SignalingPathwaySetting-upBasedonBioinformatics
9.1Methods
9.1.1MeanandVariance
9.1.2HierarchicalClustering
9.1.3CompetitiveLearningNetwork
9.1.4Self-OrganizingMap（SOM）
9.2Results
9.2.1MeanandVarianceAnalysisofCytoplasmicSignalinginControlandSTI571-TreatedK562cells
9.2.2CompetitiveLearningNetworkAnalysisofCytoplasmicSignalinginControlandSTI571-TreatedK562cells
9.2.3HierarchicalClusteringAnalysisofCytoplasmicSignalinginControlandSTI571-TreatedK562cells
9.2.4SOMAnalysisofCytoplasmicSignalinginControlandSTI571-TreatedK562cells
9.3Discussion
9.4Conclusions
References